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Image Search Results
Journal: eLife
Article Title: A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction
doi: 10.7554/eLife.44530
Figure Lengend Snippet: ( A ) Western blot images of neuregulin 1-β1 (NRG1- β1; top) and β-actin (bottom) protein expression in three-independent ESC-derived MN cultures (N1 – N3). ( B ) Bar graph quantifying qRT-PCR results for epsilon subunit of AChR (CHRNE) gene expression in 2D (light blue) and 3D (blue bars) muscle alone, NRG1- β1 treated muscle cultures, and neuromuscular co-cultures. N = 3 muscle patient donors for data presented in ( B ). In ( B ) each symbol represents data from one muscle patient donor. Values in ( B ) are mean ±SEM. *p<0.05 and **p<0.01 compared to 3D muscle alone, and ## p<0.01 compared to 2D muscle culture. ( C ) Representative GCaMP6 epifluorescence images of 3D neuromuscular co-cultures pre-treated for 3 days with healthy (top) or myasthenia gravis (MG, bottom) patient IgG, together with human complement, and then stimulated with ACh (100 μM). Scale bars, 250 μm. ( D ) Bar graph indicating the percent total 3D tissue area occupied by muscle fibers responding to acetylcholine (ACh) stimulation in healthy and MG patient IgG treated neuromuscular co-cultures. n = 4 neuromuscular tissues treated with healthy IgG and three neuromuscular tissues each treated with serum IgG from one of three separate MG patient donors. Values in ( D ) are mean ±SEM. ***p<0.001.
Article Snippet: In experiments using neuregulin1-β1 treatment,
Techniques: Western Blot, Expressing, Derivative Assay, Quantitative RT-PCR
Journal: eLife
Article Title: A 3D culture model of innervated human skeletal muscle enables studies of the adult neuromuscular junction
doi: 10.7554/eLife.44530
Figure Lengend Snippet: List of primary antibodies.
Article Snippet: In experiments using neuregulin1-β1 treatment,
Techniques: